h3Postdoctoral Researcher in the Drug Metabolism and Pharmacokinetics (DMPK) Section /h3pIdorsia Pharmaceuticals /ppBasel, Switzerland /ph3Job Responsibilities /h3ulliEstablish and evaluate New Approach Methodologies (NAMs) such as advanced in vitro hepatic systems to improve the prediction of human clearance, with a particular focus on low-turnover compounds; /liliCompare intrinsic clearance data generated in conventional systems, such as human liver microsomes and cryopreserved hepatocytes, with data from more complex liver-cell models such as HepaRG, HepatoPac, TruVivo or related systems; /liliDevelop and apply robust LC-MS/MS bioanalytical methods to quantify parent compound depletion and, where appropriate, metabolite formation in those assays; /liliInvestigate the impact of nonspecific binding, compound stability, incubation recovery and free concentration on the interpretation of clearance data and support in vitro-in vivo extrapolation in collaboration with DMPK modelers; /liliAssess the suitability of advanced hepatic systems for drug-metabolizing enzyme phenotyping in comparison with human liver microsomes, integrating metabolite identification where appropriate; /liliDocument, analyze and present experimental results in multidisciplinary project teams, and contribute to scientific reports, internal presentations, conference communications and peer-reviewed publications. /li /ulh3Candidate's Requirements /h3ulliPhD in pharmaceutical sciences, analytical chemistry, biochemistry, biology, pharmacology or a related discipline, with solid scientific training and a strong interest in applying experimental and analytical skills to DMPK questions; /liliHands-on experience with cell culture and LC-MS/MS bioanalysis is required; experience with high-resolution MS and/or metabolite identification is an advantage; /liliExperience with in vitro ADME/DMPK assays, such as microsomal or hepatocyte stability, protein binding, drug-metabolizing enzyme phenotyping, is desirable; /liliGood understanding of drug metabolism, hepatic clearance mechanisms, enzyme phenotyping and in vitro-in vivo extrapolation, or strong motivation to develop expertise in these areas; /liliProactive, collaborative and reliable personality with strong analytical thinking, excellent organizational and communication skills, and the ability to work effectively in a multidisciplinary and multicultural environment; /liliExcellent knowledge of English. /li /ul #J-18808-Ljbffr
