PHD

RNA-DNA Triple Helix formation in Chromatin Gene Regulation and Therapeutic Application The Längst Lab at the University of Regensburg is seeking a highly motivated PhD student to join our team and contribute to our cutting-edge research on RNA-DNA triple helices . About the Project Eukaryotic chromosomes are intimately associated with a pool of chromosome-associated RNA playing crucial roles in nuclear architecture, chromatin structure, and gene expression. The formation of RNA-DNA triple helices enables sequence-specific binding of caRNA to DNA, defining a novel gene regulatory mechanism with significant therapeutic potential. This DFG-funded PhD project will delve deep into the mechanism of RNA-DNA triple helix formation within cellular chromatin. You\’ll explore how nucleosome positioning and the histone code influence the formation of these triplexes, and study non-coding RNA-mediated gene regulation. The project also aims to develop a novel class of RNA therapeutics , with initial applications aimed at modulating the immune system. Your research will address: • The molecular mechanism of RNA-DNA triple helix formation within chromatin , using both reconstituted chromatin systems and mammalian cell culture. • The specific roles of these RNA-DNA interactions in regulating gene expression . • The binding affinity and stability of these structures in vitro and in vivo , utilizing a multidisciplinary approach encompassing biochemical, biophysical, and bioinformatics methods . About the Längst Lab Our research ( centers on understanding chromatin dynamics and its role in gene regulation. We are a collaborative and supportive team, committed to fostering the scientific growth of our members. The University of Regensburg provides a vibrant academic environment with excellent research facilities. We\’re looking for an enthusiastic candidate with: • A Master\’s degree in biochemistry, molecular biology, biophysics, or a related field. • A strong interest in chromatin biology, RNA biology, and gene regulation. • Hands-on experience in molecular biology, protein biochemistry, and/or biophysical techniques. • Excellent communication skills and the ability to work both independently and as part of a collaborative team. If you\’re passionate about fundamental research with significant translational implications, we encourage you to apply. Please send your application, including a cover letter outlining your motivation and research interests, your detailed CV, and contact details of two academic references, to gernot.laengst@ur.de . We look forward to hearing from you June 2025 Prof. Gernot Längst Applied Epigenetics – University of Regensburg